Innovative Methodology Quantitative Estimation of Calcium Dynamics From Ratiometric Measurements: A Direct, Nonratioing Method
نویسندگان
چکیده
Sébastien Joucla, Andreas Pippow, Peter Kloppenburg, and Christophe Pouzat Cerebral Physiology Laboratory, Université Paris-Descartes, Unité Mixte de Recherche 8118 du Centre National de la Recherche Scientifique, Paris, France; and Center for Molecular Medicine Cologne, Cologne Excellence Cluster in Aging Associated Diseases, Institute of Zoology and Physiology, University of Cologne, Cologne, Germany
منابع مشابه
Quantitative estimation of calcium dynamics from ratiometric measurements: a direct, nonratioing method.
Measuring variations of intracellular free calcium concentration through the changes in fluorescence of a calcium-sensitive dye is a ubiquitous technique in neuroscience. Despite its popularity, confidence intervals (CIs) on the estimated parameters of calcium dynamics models are seldom given. To address this issue, we have developed a two-stage model for ratiometric measurements obtained with ...
متن کاملQuantitative estimation of calcium dynamics from ratiometric
Quantitative estimation of calcium dynamics from ratiometric 1 measurements: a direct, non-ratioing, method 2 3 4 5 Sébastien Joucla , Andreas Pippow 2 , 6 Peter Kloppenburg 2 and Christophe Pouzat 7 8 9 10 1 Cerebral Physiology Laboratory, Université Paris-Descartes, CNRS, UMR 8118, 11 45 rue des Saints-Pères, 75006 Paris, France 12 13 14 2 Institute of Zoology and Physiology, Center for Molec...
متن کاملEstimation of the Elastic Properties of Important Calcium Silicate Hydrates in Nano Scale - a Molecular Dynamics Approach
Approximately, 50 to 70 percent of hydration products in hydrated cement paste are polymorphisms of C-S-H gel. It is highly influential in the final properties of hardened cement paste. Distinguishing C-S-H nano-structure significantly leads to determine its macro scale ensemble properties. This paper is dealt with nano-scale modeling. To achieve this, the most important C-S-H compounds, with a...
متن کاملEstimating weak ratiometric signals in imaging data. II. Meta-analysis with multiple, dual-channel datasets.
Ratiometric fluorescent indicators are used for making quantitative measurements of a variety of physiological variables. Their utility is often limited by noise. This is the second in a series of papers describing statistical methods for denoising ratiometric data with the aim of obtaining improved quantitative estimates of variables of interest. Here, we outline a statistical optimization met...
متن کاملA Novel, Rapid Method to Quantify Intraplatelet Calcium Dynamics by Ratiometric Flow Cytometry
Cytosolic free calcium ions represent important second-messengers in platelets. Therefore, quantitative measurement of intraplatelet calcium provides a popular and very sensitive tool to evaluate platelet activation and reactivity. Current protocols for determination of intracellular calcium concentrations in platelets have a number of limitations. Cuvette-based methods do not allow measurement...
متن کامل